Expression and homology modeling of sterol 14α-demethylase fromPenicillium digitatum
نویسندگان
چکیده
منابع مشابه
Functional analysis of two sterol regulatory element binding proteins in Penicillium digitatum
The sterol regulatory element binding proteins (SREBPs) are key regulators for sterol homeostasis in most fungi. In the citrus postharvest pathogen Penicillium digitatum, the SREBP homolog is required for fungicide resistance and regulation of CYP51 expression. In this study, we identified another SREBP transcription factor PdSreB in P. digitatum, and the biological functions of both SREBPs wer...
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Malassezia globosa cytochromes P450 CYP51 and CYP5218 are sterol 14α-demethylase (the target of azole antifungals) and a putative fatty acid metabolism protein (and a potential azole drug target), respectively. Lanosterol, eburicol and obtusifoliol bound to CYP51 with Kd values of 32, 23 and 28 μM, respectively, catalyzing sterol 14α-demethylation with respective turnover numbers of 1.7 min(-1)...
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Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis.
Sterol 14alpha-demethylase encoded by CYP51 is a mixed-function oxidase involved in sterol synthesis in eukaryotic organisms. Completion of the Mycobacterium tuberculosis genome project revealed that a protein having homology to mammalian 14alpha-demethylases might be present in this bacterium. Using genomic DNA from mycobacterial strain H(37)Rv, we have established unambiguously that the CYP51...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2007
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2007.00929.x